9926t  (Cell Signaling Technology Inc)

Journal: BioMed Research International

Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

doi: 10.1155/2022/3263446

Figure Lengend Snippet: Effects of BA on MAPK pathways in LPS-stimulated BV-2 microglia. The expression levels of p-ERK and ERK (a, b), p-JNK and JNK (c, d), and p-P38 MAPK and P38 MAPK (e, f) were analyzed by Western blot. BV-2 was incubated with BA (2.5-22.5 μ M) for 1 h before being stimulated with 0.1 μ g/mL LPS for different times (10 min for p-ERK/ERK and 40 min for p-JNK/JNK and p-P38 MAPK/P38 MAPK). Results were presented as mean ± SD ( n = 3). ### P < 0.001 vs. control; ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. LPS.

Article Snippet: MAPK family antibody sampler kit, phosphorylated MAPK family antibody sampler kit, and primary antibodies against β -actin, COX-2, IRAK1, IRAK4, MyD88, and NF- κ B P65 were purchased from Cell Signaling Technology.

Techniques: Expressing, Western Blot, Incubation, Control

Journal: BioMed Research International

Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

doi: 10.1155/2022/3263446

Figure Lengend Snippet: Mechanism of BA mitigating the neuroinflammation responses in LPS-stimulated BV-2 microglia. BA mitigates the neuroinflammation responses in LPS-stimulated BV-2 through the TLR4/MyD88/NF- κ B and MAPK pathway. BA inhibits the expression of miR-155 and release of inflammatory mediator (NO, PGE2, and IL-1 β ).

Article Snippet: MAPK family antibody sampler kit, phosphorylated MAPK family antibody sampler kit, and primary antibodies against β -actin, COX-2, IRAK1, IRAK4, MyD88, and NF- κ B P65 were purchased from Cell Signaling Technology.

Techniques: Expressing